385 Purinergic molecules in murine mast cells

In the skin, adenosine triphosphate (ATP) is released from various types of cells by environmental stimuli via nonlytic mechanisms, cell damage, or acute death. Because ATP a potent inducer skin inflammation, it has to be promptly hydrolyzed for achieve homeostasis. Of note, activates mast (MCs) through P2X7R, leading enhanced inflammation. However, MC involvement in impairment inflammation hydrolysis remains largely unknown. Thus, we sought determine expression ATP-hydrolyzing molecules MCs. Bone marrow- and fetal skin-derived MCs (BMMCs FSMCs) were used study. A culture BMMCs FSMCs with 1 mM ATP-γ-S, non-metabolizable analogue, μM ionomycin 60 min 10 min, respectively, induced their degranulation. PBS-treated steady-state strongly expressed mRNA Entpd-1 (CD39) that potently hyrdolyze ATP. While both ATP-γ-S upregulated expression. Moreover, BMMCs, FSMCs, single-cell suspension back B6 female mice clearly express at protein levels. Exogenous (1,000 nM) was added PBS- ionomycin-treated check whether on murine functional. 80% exogenous addition, tended increase hydrolyzation. These data suggest participate (CD39). degranulated trended towards activity. Collectively, functional molecules, thereby contributing